The Holy Quran is the source of knowledge, wisdom and program of prosperous life for human, and everyone takes something of it according to his/her capabilities. Poets as well have used this eternal source according to make their poems more more acceptable. Entry of the Holy Quran into Persian language caused its richness and extending of its poetic themes and making the style of Persian poetry simple. Nasimi Shirvani, poet of Timurid period, due to familiarity with the Holy Quran - undoubtedly has been affected by the text of Quran in his words. Within his poetry he sometimes has used the very same Quran verses and sometimes some parts of them are included in his poetry. In another parts of his poems, Nasimi has pointed Quranic and religious themes such as the resurrection, unity and the decline of world. Refering to prophets(PBUT), that their stories have been told are another part of Nassimi poetry. Authors of the present study are going to examine the effects of the holy Quran’s expressions, characters and stories on him and his ability and intelligence to use the holy Quran, by the use of descriptive-analytical method.